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Flow Stars

Bitesize Bio

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Candid conversations with the big hitters of flow cytometry presented by Dr Peter O'Toole. Brought to you by Beckman Coulter Life Sciences and Bitesize Bio. Flow Stars is a podcast from Beckman Coulter Life Sciences and Bitesize Bio that interviews some big hitters in the flow cytometry world. Your host is Dr Peter O'Toole (University of York), an engaging and energic personality who knows how to throw a few curveballs to keep his guest on their toes. This series takes us through the highs a ...
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"The Conversational Flow" an official podcast of KCAS Bioanalytical and Biomarker Services, and focuses on the more niche topic of Flow Cytometry and its role within the greater pharmaceutical, biopharmaceutical and bioanalytical industries.. Every month, KCAS will bring you another 60 minutes (or so) of friendly banter between our two finest Flow Cytometry experts as they chat over coffee and discuss what they've learned about the world of Flow Cytometry the past couple of weeks. The Weekly ...
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Today, Peter O'Toole chats with Dave Novo-Lake, founder of De Novo Research, a pioneering figure in flow cytometry data analysis, and the creator of FCS Express. Dave shares his journey from developing groundbreaking software in his spare time at a Boston lab to establishing a successful business that transformed clinical flow cytometry. He also ta…
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In the 19th episode of “The Conversational Flow”, our hosts, Brian and Adam, dive into the utility and innovation behind backbone panels in spectral flow cytometry, spotlighting a recent paper from BMS that presents a robust, flexible assay designed for immune monitoring using the Aurora platform. They discuss how backbone panels streamline assay d…
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Today, our host Peter O'Toole sits down with the distinguished Mario Roederer, ImmunoTechnology Section Chief and Acting Head of the Translational Research Program at the National Institutes of Health. Mario shares some fascinating insights from his illustrious career, from demonstrating flow cytometry to Presidents Barack Obama and George Bush to …
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Let's shift our focus from just the people behind the science to the technologies they're using. In this special episode, we dive deep into spectral flow cytometry with a panel of leading experts, featuring: • Tamar Tak, Coordinator Flow Cytometry Facility, Leiden University Medical Center • Domenico Lo Tartaro, University of Modena and Reggio Emil…
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In this engaging episode of the podcast, hosts Brian Wile and Adam Cotty welcome special guests Michael Naso, Hillary Quinn, and Barry Morse from The Therapeutic Innovations Group—a powerhouse team of scientists and biotech veterans with decades of experience across biologics, small molecules, and cell therapy. The conversation dives deep into the …
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Episode 17 of The Conversational Flow podcast, hosted by Brian Wile and Adam Cotty, dives into the evolving landscape of flow cytometry networking and regulatory guidance, where they analyze recent FDA biomarker validation guidance, comparing it to existing industry standards like CLSI H62. Our hosts explore the challenges of harmonizing flow cytom…
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In episode 16 of The Conversational Flow podcast, hosts Brian Wile and Adam Cotty are joined by Allie Greenplate and Ayman Rezk to explore “The Role of Flow in Drug Autoimmune Indication Research.” The discussion delves into the surge in pharmaceutical R&D for immune and autoimmune indications, highlighting the influence of oncology treatments in s…
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In this exciting 15th episode of The Conversational Flow podcast, titled "Fantastic Fluorophores and Where to Find Them," hosts Brian Wile and Adam Cotty from KCAS Bio are joined by special guest Kelly Lundsten from FluoroFinder. Together, they dive into the fascinating world of spectral flow cytometry, exploring key questions such as whether spect…
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A flow cytometry backbone panel is a set of antibodies that can be used as a starting point for identifying cell types, and then supplemented with additional markers. On the most recent episode of “The Conversational Flow”, our hosts, Brian and Adam, spend some time together as they answer the questions of “What is a backbone panel?”, “What type of…
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During the most recent episode of “The Conversational Flow” podcast, our hosts Brian and Adam talk about those times when a smaller panel might be desirable over a larger panel. To dive deep into this discussion and really analyze all the angles of these questions, the guys have invited a special guest to join them – Amrutesh Puranik, PhD. Amrutesh…
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In the 12th episode of “The Conversational Flow” podcast, Brian and Adam welcome two special guests – Sean Burke of Denovo Software and Ryan Brinkman of Dotmatics – who join them to discuss the answers to a number of specific questions about High Content Analysis: How can we manage and derive insight from higher complexity panels? How can this be s…
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During this special episode of "The Conversational Flow" our hosts, Brian and Adam, welcome two guests: John Bucksath, CEO of KCAS Bio, and Stefan Cross, CEO of Crux Laboratories. These two guests discuss the elements and advantages of their organizations' new partnership between KCAS' US and European locations and Crux's location in Melbourne, Aus…
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When it comes to flow cytometry instrumentation, there are a number of things that can be different. You may have different types of lasers, you might have a different path for the laser to follow to get to the detector, you can have different actual detectors, different filters in front of those detectors, not to mention the varying sensitivities …
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In this special episode of Flow Stars recorded live at CYTO 2024, our panel of esteemed guests, representing a cross-section of cytometry users, discuss the biggest matters in flow cytometry. They explore the capabilities of current cytometers and what future developments should focus on. They also consider the importance of making cytometry equipm…
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Let’s face it - nobody likes batch effects (unless it has to do with cookies, but that’s for another podcast). In episode #9 of “The Conversational Flow”, Brian and Adam talk about Batch Effects which is when you're having changes in the study that are not necessarily related to the scientific variables of the study – and what that might mean you w…
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For episode #8 of “The Conversational Flow” podcast, Brian and Adam spend the time discussing Receptor Occupancy Testing in Clinical Trials. Receptor occupancy (RO) assays are important in clinical trials because they provide valuable information about a drug’s binding characteristics and mechanism of action. Our hosts begin by outlining the genera…
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For episode #7 of “The Conversational Flow”, Brian and Adam invite David Ambrose to join them as they continue their conversation about Receptor Occupancy assays. There are so many unique considerations around an RO assay and with any flow cytometry assay, the precision of that assay is important. Our hosts discuss the biggest differentiators when …
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The newest episode of “The Conversational Flow” has Brian and Adam talking about the concepts around Receptor Occupancy and all the deceptively simple questions people often have about them, as well as the important but sometimes difficult answers to those questions. They talk about the biology of receptors and the few different "classes" associate…
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During this newest episode of “The Conversational Flow”, Brian and Adam discuss cell sorting and the pivotal role it plays in molecular biology applications – enabling researchers to isolate specific cell populations from complex mixtures, as well as a number of other benefits. Our hosts dive into the importance of cell sorting, the evolution of th…
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For the 4th episode of “The Conversational Flow” podcast, Brian and Adam discuss spectral flow; something really near and dear to their hearts. This is a topic both of our hosts have been genuinely interested in since they really became aware of it, at which time they immediately began pushing to bring the instrumentation to KCAS. While they have h…
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There are a number of different guidances for Flow Cytometric Assay Validation, so for episode #3 of “The Conversational Flow” podcast, Brian and Adam go into great detail on how we perform validations at KCAS, and under what context we do so – and why. In this episode, the guys break down the different types of validation and the various levels of…
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In this episode, Dr. Monika Lamba, a pathologist from Q² Solutions, discusses the transformative role of digital pathology in clinical trials. Despite its origins aiming to streamline trials, it's not yet the default. Dr. Lamba explores its evolution from telepathology and its integration into trials. The conversation covers challenges in trial org…
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#S4:E6 — Karen Hogg is a Technical Specialist at the University of York, and Margaret Mbuchi is a Principal Research Scientist at the Kenya Medical Research Institute. In this episode of Flow Stars, Karen and Margaret join Peter O’Toole to celebrate the achievements of flow cytometry initiatives across Africa and discuss some of the logistical chal…
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For our second episode of “The Conversational Flow” podcast, Brian and Adam felt that the best topic to discuss was that of sample collection, sample processing and the important role it plays in Flow Cytometry analysis. This is an often discussed topic – What is the best way to collect and process samples – and it really comes down how you plan to…
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#S4:E5 — Alexis Perez Gonzalez is the Melbourne Cytometry Platform Manager at the University of Melbourne. In this episode of Flow Stars, Alexis joins Peter O’Toole to discuss how academic progression works in Cuba and how much fun he had working with Andy Riddell. They also chat about his magnificent collection of spices and cookbooks. Watch or li…
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#S4:E4 — Ryan Brinkman is Professor Emeritus at the British Columbia Cancer Agency and Chair of the International Society for Advancement of Cytometry's (ISAC) Data Standards Task Force. In this episode of Flow Stars, Ryan joins Peter O’Toole to discuss generating data without a wet lab. They also discuss bioinformatics' clinical and translational …
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Flow cytometry is a technique used to detect and measure physical and physiological characteristics of a population of cells or particles. Tens of thousands of cells can be quickly examined and the data gathered is processed by a computer. Uses of flow cytometry can included determination of Cellular characteristics and function, biomarker detectio…
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#S4:E3 — Jessica Back is Assistant Professor - Research at the Karmanos Cancer Institute at Wayne State University. In this episode of Flow Stars, Jessica joins Peter O’Toole to discuss her difficulties getting through grad school. They also chat about places you can talk science without feeling like the odd one out and the time she had to process …
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#S4:E2 — Peter Lopez is Associate Professor in the Department of Pathology at New York University and former Director of the Cytometry and Cell Sorting Laboratory. In this episode of Flow Stars, Peter joins Peter O’Toole to discuss the benefits of semi-retirement. They also chat about his side hustle making pizza and maple syrup. Watch or listen to…
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#S4:E1 — In this special episode of Flow Stars recorded live at CYTO 2023, host Peter O'Toole welcomes a panel of distinguished experts, including Jessica Houston (New Mexico State University), Pratip Chattopadhyay (NYU Langone Health (PIL)), and Paul Wallace (Roswell Park Cancer Comprehensive Center). Join them for an enlightening conversation as …
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In this episode Dr. Monika Lamba Saini and Bruno Larvol of “LARVOL & Friends” discuss the role of artificial intelligence (AI) in pathology and its potential impact on healthcare. They delve into the slower adoption of AI in pathology compared to other medical specialties and examine the challenges that lie ahead. The conversation explores the need…
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In this episode, Megan McCausland, Scientific Advisor for Flow Cytometry at Q2 Solutions, and Scott Bornheimer, Associate Director of Medical and Scientific Affairs at BD Biosciences talk about the enormous potential of flow cytometry as a clinical diagnostic tool. Currently, pharma companies are looking for simplified, standardized flow cytometry …
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#S3:E6 — Sara De Biasi and Andrea Cossarizza from the University of Modena and Reggio Emilia (UNIMORE) chat with Peter O'Toole about how they were able to quickly respond to news of a novel coronavirus as early as January 2020, and how their work was crucial to understanding the effect of COVID-19 on the immune system. They also discuss the pros an…
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#S3:E5 — Jessica Houston chats to Peter O'Toole about developing her own niche as a new PI, while balancing this with a busy family home life. She discusses her new appointment as president-elect for the International Society for Advancement of Cytometry (ISAC), and the important role societies can play in science. She recalls her early career deve…
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#S3:E4 — Bill Telford is Head of the NCI Research Flow Facility at the NCI Center for Cancer Research. In this episode of Flow Stars, Bill joins Peter O'Toole to discuss his early career inspirations, his passion for supporting international instrument donation and training initiatives, some of the challenges he's faced running a flow core facility…
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#S3:E3 — Andrea Holme is Head of the Iain Fraser Cytometry Centre at the University of Aberdeen. In this episode of Flow Stars, Andrea joins Peter O'Toole to discuss how she was able to use her passion for traveling to her advantage during her varied scientific career. They also chat about the key differences between industry and academia, why she …
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With fewer people covering more roles with less specialization and novel therapeutics with unique testing requirements, the needs of small biotech companies in clinical trials are different from larger sponsors. In this episode, Alex Watt, Global Head for Biotech Integrated Laboratory Solutions and Mona Henderson, Director of Laboratory Network Sol…
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#S3:E2 — Grace Chojnowski is Flow Cytometry and Imaging Facility Manager at the Queensland Institute of Medical Research. In this episode of Flow Stars, Grace joins Peter O'Toole to discuss why understanding the theory behind your experiment is key in flow cytometry, why she'd like a flow lab in the Alps, and her early career as an Australian livin…
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#S3:E1 — John Tigges joins Peter O'Toole in the latest episode of Flow Stars to discuss his passion for training the next generation of scientists in flow cytometry, his hobbies of DJing and MCing, and managing the business side of a core facility. We also hear why he thinks people who work in core facilities are often like the bartenders of the sc…
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In this episode, Alex Watt, Global Head for Biotech Integrated Laboratory Solutions and Alan Wookey, Global Head of Companion Diagnostics discuss how to manage both scientific and operational risk in clinical trials for biotech companies. Looking at risk as uncertainty reveals not only threats to project timelines but also opportunities to take a n…
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Alex Watt is the Global Head for Biotech Integrated Laboratory Solutions at Q2 Solutions. In this episode, the first of a three-part series, he talks about how biotech companies can best prepare for testing with a CRO or clinical trial. First it's important to understand you analytical needs. What services are needed to operationalize the study des…
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#S2:E6 — Paul K. Wallace of the Roswell Park Comprehensive Cancer Center was one of the first clinical cytometrists. He has had a huge influence on the field of flow cytometry, particularly in developing assays to track immune function. In this engaging episode of Flow Stars, Paul chats to Peter O’Toole about his early ambitions to become a vet, th…
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Becoming bilingual in bioanalysis In this podcast Barry Jones (Director, LC–MS Biologics and Biomarkers at Q² Solutions) and Adriane Spytko (Manager, LC–MS Biologics at Q² Solutions; both NY, USA) discuss hybrid assays using both LBA and LC−MS techniques. They share their thoughts on bottom-up LC−MS methods and the concern about detecting analyte t…
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#S2:E5 — Alice Givan, now retired, was previously the director of the Flow Cytometry/Fluorescence Imaging Core at the Norris Cotton Cancer Center/Dartmouth Medical School in New Hampshire and author of the classic text Flow Cytometry: First Principles. In today's episode of Flow Stars, Alice joins Peter O'Toole to discuss how funding issues led to …
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#S2:E4 — Rui Gardner is Flow Cytometry Core Head at the Memorial Sloan Kettering Cancer Center. In today’s lively episode of Flow Stars, Rui joins Peter O’Toole to discuss why all undergraduates should be taught how to ask questions, science careers for extroverts, conference nostalgia—and pitchers of sangria—and how to take a brooding boyband lab …
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#S2:E3 — Kylie Price is the Head of Research Technology at the Malaghan Institute of Medical Research in New Zealand and current Secretary of ISAC. She is a tireless proponent of the benefits of cytometry and of improving science and technology in New Zealand. She’s also pretty tireless in general, it turns out, as we discover in this episode of Fl…
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#S2:E2 — In the latest episode of Flow Stars, Peter O’Toole is joined by the inspiring Pratip Chattopadhyay, who has had an illustrious career in the fields of flow cytometry and immunoassays and was the founding Director of NYU Langone Health’s Precision Immunology Laboratory (PIL). He is also the founder of startup biomarker discovery engine Talo…
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#S2:E1 — Garry Nolan of the Stanford University School of Medicine has been hugely influential in the fields of hematopoiesis, cancer and leukemia, and network and systems immunology. In the latest inspiring episode of Flow Stars, Garry chats to Peter O’Toole about ensuring scientific research benefits the public, his collection of carnivorous plan…
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Most clinical trials involve a variety of laboratory vendors and CROs to provide services during the course of the study. Disparity in how each vendor handles, tracks and reports its data can present a challenge. In this episode, Barbara Nagaraj, Senior IT Architect at Biofortis, a Q2 Solutions company, describes those challenges and how to mitigat…
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Taking an assay from bench to an automated platform In this podcast, Prakash Bhosale (Director of Discovery ADME at Q2 Solutions; IN, USA) discusses high-throughput in vitro screening and how to understand structure-activity relationships against ADME properties. The advantages of the Tecan technology are also addressed as well as top tips for driv…
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