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DNA on the move – the influence of form on gel migration

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Manage episode 463705217 series 3466726
Content provided by Thermo Fisher Scientific. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Thermo Fisher Scientific or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://ppacc.player.fm/legal.

Agarose gel electrophoresis is a staple method in almost all biology and biochemistry lab where separation and analysis of nucleic acids is needed. In this Mol Bio Minutes mini episode Augustė Užuotaitė, Scientist III at Thermo Fisher Scientific, covers the basics of electrophoresis with a spotlight on how different forms of DNA migrate differently in agarose gel electrophoresis.

In less than 10 quick minutes, you’ll learn about the many factors that affect DNA migration rate. Augustė reviews how DNA size, sequence, and conformation all affect migration rate, and she gives some beautifully simple examples to help it all make sense.

Helpful resource links mentioned in this episode:

Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. 

Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.

For Research Use Only. Not for use in diagnostic procedures.

  continue reading

30 episodes

Artwork
iconShare
 
Manage episode 463705217 series 3466726
Content provided by Thermo Fisher Scientific. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Thermo Fisher Scientific or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://ppacc.player.fm/legal.

Agarose gel electrophoresis is a staple method in almost all biology and biochemistry lab where separation and analysis of nucleic acids is needed. In this Mol Bio Minutes mini episode Augustė Užuotaitė, Scientist III at Thermo Fisher Scientific, covers the basics of electrophoresis with a spotlight on how different forms of DNA migrate differently in agarose gel electrophoresis.

In less than 10 quick minutes, you’ll learn about the many factors that affect DNA migration rate. Augustė reviews how DNA size, sequence, and conformation all affect migration rate, and she gives some beautifully simple examples to help it all make sense.

Helpful resource links mentioned in this episode:

Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. 

Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.

For Research Use Only. Not for use in diagnostic procedures.

  continue reading

30 episodes

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