Fast PCR fundamentals – from instruments to enzymes

Speaking of Mol Bio

In this Mol Bio Minutes episode, Laurynas Alijošius breaks down how to run fast PCR to save time and increase lab efficiency. He explains how to choose the right thermal cycler with fast ramp speeds, select low-volume and thin-walled PCR plastics, and use engineered DNA polymerases that offer rapid elongation and hot-start capability. Laurynas also covers practical tips for optimizing reaction components, shortening cycling protocols, and reducing waste. Whether you’re aiming to finish your experiment before dinner or streamline your workflow long-term, this episode delivers everything you need to master the art of fast PCR.

Helpful resource links mentioned in this episode:

• Molecular biology handbook – An extensive resource for all things molecular biology
• Applied Biosystems thermal cyclers – Including those that support fast PCR
• PCR consumables – Four key attributes to consider
• PCR plastics selection tool – Find the right plastics for your instrument and fast PCR
• DNA polymerases – Four key characteristics to know and consider
• PCR setup optimization – Six critical things to optimize for optimal PCR results
• Enzymes and master mixes – Get the right reagents to drive your PCR reaction
• Cycling optimization – Instrument considerations for PCR success

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For Research Use Only. Not for use in diagnostic procedures.