))
High-Throughput and Wide-Range Protein Concentration Determination of Monoclonal Antibodies
Manage episode 468206826 series 3391756
Content provided by Bitesize Bio. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Bitesize Bio or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://ppacc.player.fm/legal.
In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings.
Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations.
Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method.
Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate.
Larger batches would require multiple analysts for a single-day turnaround time.
With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential.
Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements.
It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL.
Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
139 episodes
Share
