))
Practical Applications and Considerations of Phenol-Chloroform Extraction
Manage episode 397068889 series 3391757
Content provided by Bitesize Bio. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Bitesize Bio or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://ppacc.player.fm/legal.
#94 — While there are lots of methods to choose from for cleaning up your RNA or DNA samples, for many researchers, phenol-chloroform is the go-to technique.
In this episode, go beyond the basics of how the method works and get expert practical guidance on performing and optimizing it.
Plus, learn the differences between the common solvents, how to check and adjust the pH of the phenol phase, and get tips to reduce the amount of interphase.
Check out the corresponding online article for a diagram illustrating how you can reduce the interphase. [1] Learn the theory behind the technique in our easy explainer [2] and explore four other ways you can clean up DNA samples. [3]
Resources:
1. Practical Application of Phenol-Chloroform Extraction. Available at: https://bitesizebio.com/3651/practical-application-of-phenol-chloroform-extraction/
2. The Basics: How Phenol Extraction of DNA Works. Available at: https://bitesizebio.com/384/the-basics-how-phenol-extraction-works/
3. Five Ways to Clean Up A DNA Sample. Available at: https://bitesizebio.com/142/5-ways-to-clean-up-a-dna-sample/
115 episodes
Share
