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5 Ingredients for the Perfect Protein Purification Buffer
Manage episode 378664301 series 3391757
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#79 — Are you struggling to keep your proteins "happy" and active for your experiments? In this episode of Mentors At Your Benchside, we dive into the five critical elements you need to design the ideal protein purification buffer: pH, the buffer system, salt concentration, reducing agents, and stabilizing additives. [1]
Learn how buffers work, [2] and how to navigate the delicate balance of these factors to prevent protein aggregation and keep your purified proteins soluble and active. [3] Tune in for a simplified guide to mastering your protein purification buffer.
Resources:
1. 5 Ingredients for the Perfect Protein Purification Buffer. Available at: https://bitesizebio.com/7893/how-to-design-the-perfect-protein-purification-buffer/
2. How Do Buffers Work? Available at: https://bitesizebio.com/8478/how-do-buffers-work/
3. The Definitive Guide to pH, pKa, and pI. Available at: https://bitesizebio.com/9425/what-is-ph-pka-pi/
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