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How FRET Works: A Guide to Visualizing Protein Interactions

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Manage episode 378038500 series 3391757
Content provided by Bitesize Bio. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Bitesize Bio or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://ppacc.player.fm/legal.

#78 — Want to visualize if your proteins interact in live cells? FRET is the answer. In this Mentors at Your Benchside episode, we explain how FRET works, why it's great for studying protein–protein interactions, and why it is not actually named Fluorescence Resonance Energy Transfer.

Visit the original article to see helpful diagrams, [1] brush up on how fluorescent molecules work, [2] and read up on how to measure FRET for the different methods and practical tips for choosing FRET pairs. [3]

Resources:
1. How FRET Works: A Guide to Visualizing Protein Interactions. Available at: https://bitesizebio.com/23012/how-fret-works/
2. How Fluorescent Molecules Work. Available at: https://bitesizebio.com/32973/fluorescent-molecules/
3. How to Measure FRET. Available at: https://bitesizebio.com/23295/how-to-measure-fret/

  continue reading

115 episodes

Artwork
iconShare
 
Manage episode 378038500 series 3391757
Content provided by Bitesize Bio. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Bitesize Bio or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://ppacc.player.fm/legal.

#78 — Want to visualize if your proteins interact in live cells? FRET is the answer. In this Mentors at Your Benchside episode, we explain how FRET works, why it's great for studying protein–protein interactions, and why it is not actually named Fluorescence Resonance Energy Transfer.

Visit the original article to see helpful diagrams, [1] brush up on how fluorescent molecules work, [2] and read up on how to measure FRET for the different methods and practical tips for choosing FRET pairs. [3]

Resources:
1. How FRET Works: A Guide to Visualizing Protein Interactions. Available at: https://bitesizebio.com/23012/how-fret-works/
2. How Fluorescent Molecules Work. Available at: https://bitesizebio.com/32973/fluorescent-molecules/
3. How to Measure FRET. Available at: https://bitesizebio.com/23295/how-to-measure-fret/

  continue reading

115 episodes

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